The Antidiabetic and Regenerative Effects of Double stem cell aqueous extract (Malus domestica) in Alloxan-induced Diabetic Rats

Double stem cell extract and glucose regulation in rats

Abstract

Diabetes Mellitus (DM) cannot be divorced from consequent dire health challenges with a projected prediction of the afflicted population expected to rise to 783 million by 2045. This study evaluated the acclaimed antidiabetic effect of aqueous extract of Double Stem Cell^® (DSC) in alloxan-induced diabetic rats. Adult male rats were randomly assigned into six groups of five animals. Group I served as non-diabetic control while groups II-VI were induced with diabetes using 150 mgkg-1, b.w, i.p of alloxan. Once daily administration of Malus domestica (50, 100, or 200 mgkg-1, p.o.), normal saline or glibenclamide was done for 21 days. Fasting blood glucose (FBG) was recorded on days 1 and 21 after establishing diabetes mellitus using the alloxan model, followed by an assessment of biochemical and haematological parameters. The liver and pancreas were harvested for histopathology and antioxidants. Alloxan administration significantly increased FBG, indicating hyperglycaemia. Post-treatment with all doses of the extract and glibenclamide resulted in a significant (p<0.001; p < 0.0001) reduction in FBG. Attenuation of alloxan-induced elevated Malondialdehyde (MDA), enhanced catalase, superoxide dismutase activities and regeneration of the alloxan-induced cell damage was demonstrated. Double Stem Cell® extract achieved hypoglycaemia and interestingly, regeneration of cytoarchitecture following alloxan-induced damage was established.